Enzyme-Linked Immunosorbent Assay (ELISA) is a powerful laboratory technique used to detect and quantify proteins, antibodies, and antigens. It is widely used for diagnosing infectious diseases caused by bacteria, viruses, and parasites. The method is favored for its sensitivity, specificity, and ability to process multiple samples simultaneously. Principle of ELISA ELISA involves the following basic … Read more
Tabulation of data refers to the systematic arrangement of data in rows and columns. This layout allows for the easy comparison and understanding of data, serving as a foundation for statistical analysis. It is a key step in organizing data into a concise format to extract insights efficiently. In biostatistics, data analysis is a structured … Read more
Diagrammatic representation of data is a method of presenting data visually using shapes, symbols, and illustrations. Unlike detailed numeric or textual data, diagrams provide a simplified view that highlights patterns, relationships, or differences in the data. as we describe earlier the classification of data in biostatistical studies. this chapter is like extension of classified data … Read more
T cell receptors (TCRs) are critical components of the adaptive immune system. They are responsible for recognizing antigens, which are specific molecules or fragments from pathogens (like viruses and bacteria) that trigger an immune response. Understanding TCRs is essential for grasping how T cells function in immune surveillance and response. Imagine a battleground where microscopic … Read more
Somatic hybridization is the technique of hybrid production through the fusion of isolated somatic(body) protoplasts under in vitro conditions and subsequent development of their product (heterokaryon) to a hybrid plant. This involves – A. Fusion of protoplast cells – 1. Treatment with NaNO3 – 1ST Rerport by power et al., 1970. In this method NaNO3 … Read more
Previously we have about of plant tissue culture (PTC) for the rapid multiplication. In PTC we can use various parts such shoot tip for shoot tip culture, leaf, etc. as explant. Imagine producing thousands of identical, healthy plants from just a small piece of tissue using PTC technique. Now let think about the plant and … Read more
To understand the protoplast isolation, lets Imagine being able to take two completely different plants—one resistant to drought, the other producing high-yield crops—and fusing them into a single super-plant. What if you could manipulate individual cells and create hybrids that nature never could? Welcome to the fascinating world of protoplast isolation, where we strip cells … Read more
What is Chloroplast Transformation? Chloroplast transformation refers to the genetic modification of chloroplast DNA (cpDNA) to express foreign genes. This process involves introducing exogenous genes into the chloroplast genome using specific vectors. Unlike nuclear transformation, which targets the nuclear DNA, chloroplast transformation directly alters the chloroplast’s genome, allowing for the production of specific proteins within … Read more
Imagine we want to study how plants grow in space or create plants with super resistance to diseases. But growing full plants can be slow, space-consuming, and hard to control. This is where callus culture come in. They allow us to grow plant cells in a lab under controlled conditions, making it easier to understand … Read more
Imagine biting into a perfectly ripe, juicy tomato. Now, imagine that same tomato, but it stays fresh and delicious for weeks rather than days. What if farmers could consistently grow fruits and vegetables that not only taste better but also last longer, reducing waste and boosting food security? Quality in agriculture isn’t just about taste; … Read more